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| Registros recuperados : 79 | |
| 5. |  | SCAGLIUSI, S. M. M.; CIMA, F. F. Otimização de protocolo para produção de plantas duplo-haploides de trigo através da cultura de micrósporos isolados. In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 8.; SEMINÁRIO TÉCNICO DO TRIGO, 9., 2014, Canela; REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 9.; SEMINÁRIO TÉCNICO DO TRIGO, 10., 2015, Passo Fundo. Anais... Passo Fundo: Biotrigo Genética: Embrapa Trigo, 2015. 2014 - Melhoramento, Aptidão industrial e Sementes - Trabalho 97. 1 CD-ROM.| Biblioteca(s): Embrapa Trigo. |
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| 8. | | BRAMMER, S. P.; SCAGLIUSI, S. M. M.; YAMAZAKI-LAU, E. Biotecnologia. In: DE MORI, C.; ANTUNES, J. M.; FAE, G. S.; ACOSTA, A. da S. (Ed.). Trigo: o produtor pergunta, a Embrapa responde. Brasília, DF: Embrapa, 2016. cap. 12, p. 237-246.| Biblioteca(s): Embrapa Trigo. |
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| 11. | | ELIAS, A. G.; SCAGLIUSI, S. M. M.; BERTOLDI, M.; GREGOSKI, C.; MINELLA, E. Albinismo: um fenômeno recorrente na obtenção de plantas duplo-haploides de cevada entre os anos de 2010 a 2014. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 10., MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 7., 2015, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2015. Graduação. p. 21.| Biblioteca(s): Embrapa Trigo. |
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| 12. | | POERSCH-BORTOLON, L. B.; SCAGLIUSI, S. M. M.; LAU, E. Y.; BODANESE-ZANETTINI, M. H. Androgenic response of brazilian wheat genotypes to different pretreatments of spikes and to a gellinhg agent. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 51, n. 11, p. 1839-1847, nov. 2016. Título em português: Resposta androgênica de genótipos brasileiros de trigo a diferentes pré-tratamentos das espigas e a um agente gelificante.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 13. | | POERSCH-BORTOLON, L. B.; SCAGLIUSI, S. M. M.; YAMAZAKI-LAU, E.; BODANESE-ZANETTINI, M. H. Androgenic response of brazilian wheat genotypes to different pretreatments of spikes and to a gellinhg agent. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 51, n. 11, p. 1839-1847, nov. 2016.| Biblioteca(s): Embrapa Trigo. |
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| 17. | | BERTOLDI, M.; SCAGLIUSI, S. M. M.; ELIAS, A. G.; GREGOSKI, C.; MINELLA, E. Duplicação cromossômica em plantas haploides de cevada: mecanismo de sobrevivência a ser seguido. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 10., MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 7., 2015, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2015. Graduação. p. 25.| Biblioteca(s): Embrapa Trigo. |
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| 20. | | COELHO, M. B.; SCAGLIUSI, S. M. M.; LIMA, M. I. P. M.; GRANDO, M. F. Efeito do pré-tratamento de espigas na indução da androgênese em trigo. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 11.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 8., 2016, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2016. p. 48.| Biblioteca(s): Embrapa Trigo. |
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| Registros recuperados : 79 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
10/12/2014 |
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Data da última atualização: |
01/02/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SCAGLIUSI, S. M. M. |
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Afiliação: |
SANDRA MARIA MANSUR SCAGLIUSI, CNPT. |
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Título: |
Establishing isolated microspore culture to produce doubled haploid plants in Brazilian wheat (Triticum aestivum L.). |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Australian Journal of Crop Science, v. 8, n. 6, p. 887-894, 2014. |
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ISSN: |
1835-2707 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to establish an isolated microspore culture (IMC) protocol in wheat (Triticum aestivum L.) for use in genetic studies and to evaluate its potential for routine use in the Brazilian Wheat Breeding Program at Embrapa Wheat. Important steps of the method were identified and plant physiology of microspore mother plants and ovary co-culture were considered as key factors for effective establishment. Three Brazilian wheat genotypes were tested (Toropi, BRS 194 and F1 wheat cross 020037 × 020062), and two other genotypes were used as controls (Bobwhite and Fielder). Spikes containing uninucleated microspores were subjected to cold pretreatment (4°C) for 21 days in the dark. Number of embryos, green and albino plants were recorded for each genotype. The method was successfully established, and several fertile green plants were produced by using tissue culture and responsive controls. However, the results greatly differed among Brazilian wheat genotypes, suggesting a strong genotype-dependent effect. Microspore induction medium alone did not promote embryogenesis; ovary co-culture was a necessary step for embryo development and green plant formation, for all genotypes. The F1 wheat cross (020037 × 020062) produced a total of 85 green plants (out of 108 spikes), 64% of which were spontaneous diploids. BRS 194 produced many embryos, exhibiting a good androgenic response, but only a few grew into green plants. Toropi behaved as a recalcitrant genotype, and zero plants were produced. To our knowledge, this is the first report on wheat IMC from Brazilian genotypes resulting in androgenic embryogenesis and plant regeneration. MenosThe objective of this study was to establish an isolated microspore culture (IMC) protocol in wheat (Triticum aestivum L.) for use in genetic studies and to evaluate its potential for routine use in the Brazilian Wheat Breeding Program at Embrapa Wheat. Important steps of the method were identified and plant physiology of microspore mother plants and ovary co-culture were considered as key factors for effective establishment. Three Brazilian wheat genotypes were tested (Toropi, BRS 194 and F1 wheat cross 020037 × 020062), and two other genotypes were used as controls (Bobwhite and Fielder). Spikes containing uninucleated microspores were subjected to cold pretreatment (4°C) for 21 days in the dark. Number of embryos, green and albino plants were recorded for each genotype. The method was successfully established, and several fertile green plants were produced by using tissue culture and responsive controls. However, the results greatly differed among Brazilian wheat genotypes, suggesting a strong genotype-dependent effect. Microspore induction medium alone did not promote embryogenesis; ovary co-culture was a necessary step for embryo development and green plant formation, for all genotypes. The F1 wheat cross (020037 × 020062) produced a total of 85 green plants (out of 108 spikes), 64% of which were spontaneous diploids. BRS 194 produced many embryos, exhibiting a good androgenic response, but only a few grew into green plants. Toropi behaved as a recalcitrant genotype, an... Mostrar Tudo |
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Palavras-Chave: |
Brasil; Duplo haplóide; Microspore culture; Wheat breeding. |
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Thesagro: |
Melhoramento Genético Vegetal; Trigo. |
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Thesaurus NAL: |
androgenesis. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/113534/1/2014-Australian-Journal-of-Crop-Science-v8n6p887.pdf
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Marc: |
LEADER 02353naa a2200217 a 4500
001 2002262
005 2015-02-01
008 2014 bl uuuu u00u1 u #d
022 $a1835-2707
100 1 $aSCAGLIUSI, S. M. M.
245 $aEstablishing isolated microspore culture to produce doubled haploid plants in Brazilian wheat (Triticum aestivum L.).$h[electronic resource]
260 $c2014
520 $aThe objective of this study was to establish an isolated microspore culture (IMC) protocol in wheat (Triticum aestivum L.) for use in genetic studies and to evaluate its potential for routine use in the Brazilian Wheat Breeding Program at Embrapa Wheat. Important steps of the method were identified and plant physiology of microspore mother plants and ovary co-culture were considered as key factors for effective establishment. Three Brazilian wheat genotypes were tested (Toropi, BRS 194 and F1 wheat cross 020037 × 020062), and two other genotypes were used as controls (Bobwhite and Fielder). Spikes containing uninucleated microspores were subjected to cold pretreatment (4°C) for 21 days in the dark. Number of embryos, green and albino plants were recorded for each genotype. The method was successfully established, and several fertile green plants were produced by using tissue culture and responsive controls. However, the results greatly differed among Brazilian wheat genotypes, suggesting a strong genotype-dependent effect. Microspore induction medium alone did not promote embryogenesis; ovary co-culture was a necessary step for embryo development and green plant formation, for all genotypes. The F1 wheat cross (020037 × 020062) produced a total of 85 green plants (out of 108 spikes), 64% of which were spontaneous diploids. BRS 194 produced many embryos, exhibiting a good androgenic response, but only a few grew into green plants. Toropi behaved as a recalcitrant genotype, and zero plants were produced. To our knowledge, this is the first report on wheat IMC from Brazilian genotypes resulting in androgenic embryogenesis and plant regeneration.
650 $aandrogenesis
650 $aMelhoramento Genético Vegetal
650 $aTrigo
653 $aBrasil
653 $aDuplo haplóide
653 $aMicrospore culture
653 $aWheat breeding
773 $tAustralian Journal of Crop Science$gv. 8, n. 6, p. 887-894, 2014.
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Embrapa Trigo (CNPT) |
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